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Effect of PRP-1 on Src and p-Src. Rabbit polyclonal anti-p-Src (Tyr416) and rabbit polyclonal anti-Src antibodies were applied to the membranes at 1:1,000, whereas goat anti-rabbit IgG peroxidase conjugate was used at 1:5,000. Mouse monoclonal anti-tubulin antibody was used at 1:2,000 and secondary anti-mouse IgG at 1:5,000. On both gels tubulin band was detected at 55 kDa.
