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. 2015 Jun 18;47(2):465–472. doi: 10.3892/ijo.2015.3054

Figure 8.

Figure 8

Effect of PRP-1 on the expression of p27 and CDK2 in human JJ012 chondrosarcoma cell line CDK2. Rabbit monoclonal anti-p27 Kip1 (D69C12) and rabbit polyclonal anti-CDK/2 both were used at 1:1,000 dilution in western blocker solution, whereas secondary antibodies goat anti-rabbit IgG peroxidase conjugate was used at 1:5,000. P27 band was detected at 27 kDa and cdk2 at 37 kDa.