Figure 8.
Effect of PRP-1 on the expression of p27 and CDK2 in human JJ012 chondrosarcoma cell line CDK2. Rabbit monoclonal anti-p27 Kip1 (D69C12) and rabbit polyclonal anti-CDK/2 both were used at 1:1,000 dilution in western blocker solution, whereas secondary antibodies goat anti-rabbit IgG peroxidase conjugate was used at 1:5,000. P27 band was detected at 27 kDa and cdk2 at 37 kDa.