Figure 5.

Expression levels of uncoupling protein (UCP)-1, UCP-2 and glucose transporter type 4 (GLUT4) in differentiated 3T3-L1 adipocytes following treatment with the tuna-derived peptide, D-I-V-D-K-I-E-I (TP-D). Cells were treated with TP-D (125, 250, 500 or 1,000 ng/ml) for 48 h. (A) Protein expression levels were measured by western blot analysis. (B) cDNA was subjected to RT-PCR, and mRNA expression was analyzed by electrophoresis on a 1% (w/v) agarose gel and staining with RedSafe nucleic acid staining solution.