Figure 3.

17-ABAG induces the degradation of Hsp90 client proteins. (A) LNCaP cells were cultured in the presence of 17-ABAG at the indicated concentrations for 24 h, and the proteins regulated by Hsp90 or ARs were assessed by western blot analysis. β-actin was used for normalization and verification of protein loading. (B) LNCaP cells were treated with 1 μM 17-ABAG for the indicated times and the proteins regulated by Hsp90 or ARs were assessed by western blot analysis. β-actin was used for normalization and verification of protein loading. HSP, heat shock protein; AR, androgen receptor; PSA, prostate-specific antigen; EGFR, epidermal growth factor receptor; Cdk4, cyclin-dependent kinase 4; Her2, human epidermal growth factor receptor 2; Nkx-3.1, Nk3 homeobox 1.