Figure 4.

17-ABAG inhibits the AR pathway in LNCaP cells. (A) LNCaP cells were treated with 0.2 μM 17-ABAG for 0 or 24 h, followed by treatment with 1 nM R1881, a potent non-aromatizable androgen, for 6 h. The nuclei were stained with Hoechst 33258 and AR localization was assessed using immunofluorescence (magnification, ×400). (B) LNCaP cells were treated with 1 μM 17-ABAG for 12 or 24 h, and PSA, FKBP5 and NKX3.1 mRNA levels were determined using quantitative real-time polymerase chain reaction. The mRNA levels were normalized to GAPDH mRNA. Values are expressed as the mean ± standard error of the mean. All of the experiments were repeated at least three times. ^**P<0.01 vs. Ctl. Ctl, control; AR, androgen receptor.