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Western blot analysis to determine caspase activation. The activities of (A) caspase-3, (B) caspase-8 and (C) caspase-9 were analyzed on day 3 in the control (lane 1), 0.794 mM APAP-treated (lane 2) and 7.94 mM APAP-treated (lane 3) cells. Ceaved caspase-3 and cleaved caspase-9 were markedly increased in the 7.94 mM APAP-treated cells. APAP, acetaminophen; hMSC, human mesenchymal stem cell.
