Table I.
Primer sequences used for using reverse transcription polymerase chain reaction to validate the microarray analysis.
| Gene | Primer sequence | Product (bp) |
|---|---|---|
| TIPE2 | F: 5′-GGGAACATCCAAGGCAAG-3′ R: 5′-AGCTCATCTAGCACCTCACT-3′ |
195 |
| Fas | F: 5′-GACCCAGAATACCAAGTGCAAGTG-3′ R: 5′-CTTGCCCTCCTTGATGTTATTTTC-3′ |
400 |
| FasL | F: 5′-CGTGAGTTCACCAACCAAAGC-3′ R: 5′-CCCAGTTTCGTTGATCACAAG-3′ |
219 |
| Bcl-2 | F: 5′-TTCTCCTTCCAGCCTGAGAGCAA-3′ R: 5′-ATGACCCCACCGAACTCAAAG-3′ |
317 |
| β-actin | F: 5′-GACTACCTCATGAAGATCCTCACC-3′ R: 5′-TCTCCTTAATGTCACGCACGATT-3′ |
190 |
F, forward, R, reverse.