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. 2015 Jun 22;47(2):719–727. doi: 10.3892/ijo.2015.3060

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Identity of normal fibroblasts (NFs) and cancer associated fibroblasts (CAFs) isolated from the tumor bearing TRAMP mice. Characteristics of NFs and CAFs. NFs and CAFs were isolated from normal and cancer tissues and immortalized using SV40-T antigen incorporation. (A) Real-time PCR results show the obtained NFs and CAFs differentially express CAF specific markers, including CD90, IGF-1 and α-SMA. (B) Carton shows our protocols to do the growth and invasion assays. (C) The capabilities of NFs and CAFs to promote PCa cell growth are different in MTT assays. (D) BrdU staining shows the capability difference of NFs and CAFs to promote PCa cell growth. (E) The capabilities of NFs and CAFs to promote PCa cells invasion are different in transwell assays. ^*p<0.05.