Figure 2.

Co-culture of BM-MSCs and NFs changes the characteristics of NFs. The NF characteristics were altered when co-cultured with the BM-MSCs. NFs were co-cultured, with or without mouse primary BM-MSCs, which had been isolated from the normal B6 mice and cultured in transwell plates for 7 days. (A) The experimental protocol. (B) Total RNAs were then obtained from the NFs. The expression of CAF markers in NFs treated by BM-MSCs (MSCs) conditional media and control regular media were analyzed by real-time PCR. (C) Total proteins were extracted from NFs (BM-MSCs pretreated and control media-treated) for western blot analysis.