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. 2015 Jun 22;47(2):719–727. doi: 10.3892/ijo.2015.3060

Figure 5.

Figure 5

Mechanism by which BM-MSCs alter the NF characteristics. (A) The strategy of extracting total RNA from BM-MSCs after co-culture with NFs. or BM-MSCs alone, which were used as a control. (B) Real-time PCR assay shows TGF-β1, IGF-1 and PDGFA mRNA expression levels are increased in mouse BM-MSCs upon co-cultured with mouse NFs. (C) For western blot assay, NF cells were treated with TGF-β1 or (and) IGF-1 for 3 days, then total protein from NFs was extracted. The protein expression of α-SMA in NFs, the major marker of CAF, was increased after treated with TGF-β1 but not IGF-1. (D) When inhibitor of TGF-β1 was added into the co-culture system, α-SMA was lightly decreased, showing the NFs to CAFs conversion was partially blocked. (E) Immunofluorescence results showing the expression of α-SMA after different treatments. (F) Co-cultured TRAMP-C1 cells with differently pre-treated NFs. The TRAMP-C1 cells were collected for the invasion assay. Quantitation is shown on the right. *p<0.05.