TABLE 2.
miR-24, miR-30b, and miR-142-3p regulate components of the TLR signaling complex
| Culture | TLR4 (% +) |
MD-1 (% +) |
TLR4 (% +) + LPS |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mφ | DC | Mφ | DC | Mφ | DC | |||||||
| Untreated | 97.5 | ns | 57.5 | ns | 90.9 | ns | 86.6 | ns | 38.9 | ns | 22.6 | ns |
| Control mimic | 96.7 | ns | 59.3 | ns | 92.0 | ns | 88.4 | ns | 29.3 | ns | 19.4 | ns |
| miR-24 mimic | 84.0 | ns | 57.6 | ns | 71.0 | ns | 77.9 | ns | 35.5 | ns | 27.4 | ns |
| miR-24 inhibitor | 83.4 | ns | 64.8 | ns | 82.9 | ns | 94.5 | ns | 40.3 | ns | 29.0 | ns |
| miR-30b mimic | 64.9 | * | 42.2 | * | 47.5 | * | 70.9 | * | 22.1 | * | 18.1 | ns |
| miR-30b inhibitor | 94.9 | ns | 69.2 | ns | 75.4 | ns | 90.9 | ns | 88.0 | * | 42.2 | * |
| miR-142-3p mimic | 63.8 | * | 38.0 | * | 28.3 | ** | 65.3 | * | 18.2 | ** | 28.5 | ns |
| miR-142-3p inhibitor | 93.4 | ns | 62.4 | ns | 79.4 | ns | 95.3 | ns | 91.0 | * | 55.3 | * |
mD-Mφ and mD-DC cultures were transfected on day 3 and analyzed by flow cytometry for TLR4 and MD-1 expression on day 7 or 8, with LPS stimulation on day 7. Data are presented as means from 5 biologic donors.
*
P < 0.05 is considered significant; **P < 0.005.