TABLE 3.
miR-24, miR-30b, and miR-142-3p target PKCα and NF-κB activation
| Culture | PKCα (% +) |
NF-κBp65 (% +) |
||||||
|---|---|---|---|---|---|---|---|---|
| Mφ | DC | Mφ | DC | |||||
| Untreated | 89.1 | ns | 80.0 | ns | 90.9 | ns | 91.2 | ns |
| Control mimic | 87.3 | ns | 85.8 | ns | 92.1 | ns | 90.9 | ns |
| miR-24 mimic | 63.3 | * | 81.4 | ns | 63.7 | * | 48.6 | * |
| miR-24 inhibitor | 86.5 | ns | 83.3 | ns | 93.5 | ns | 92.9 | ns |
| miR-30b mimic | 60.8 | * | 49.2 | * | 64.0 | * | 29.0 | * |
| miR-30b inhibitor | 89.0 | ns | 83.7 | ns | 91.8 | ns | 88.6 | ns |
| miR-142-3p mimic | 56.3 | * | 33.2 | * | 31.5 | ** | 4.4 | * |
| miR-142-3p inhibitor | 84.2 | ns | 87.5 | ns | 90.7 | ns | 93.2 | ns |
mD-Mφ and mD-DC cultures were transfected on day 3 and PKCα activation analyzed by flow cytometry on day 5. Data are presented as means and sd from 3 biologic donors. mD-Mφ and mD-DC cultures were transfected on day 3, stimulated with LPS on day 7, and analyzed for NF-κBp65 expression after 4 h by flow cytometry. Data are presented as means from 5 biologic donors.
*
P < 0.05 is considered significant; **P < 0.005.