FIG. 5.

LNX2 inhibits the proliferation but promotes neuronal differentiation of NPCs. (A) NPCs were transfected with LNX2 siRNA and its scrambled siRNA (NC), respectively. Transfected cells were stained for BrdU and DAPI after being cultured in the medium containing BrdU for 3 h. The numbers of BrdU⁺ cells were counted and expressed as the percentage of the number of DAPI⁺ cells (NC: 39.68±1.39; LNX2 siRNA: 55.23±1.75). (B, C) NPCs were transfected with LNX2 cDNA in a pCDF1-MCS1-EF1-copGFP vector expressing coGFP [LNX2, (B)] or in an empty vector containing only copGFP [GFP, (B)] and a LNX2 shRNA [LNX2 shRNA, (C)] or a scrambled shRNA as a control [NC, (C)]. After 3–4 days of differentiation in vitro, cells were double-stained for TUJ1, GFP, and DAPI. The numbers of GFP⁺TUJ1⁺ cells were counted and expressed as the percentage of the number of GFP⁺ cells. (B) Control: 15.33±1.01; LNX2: 20.76±0.77; (C) Control: 48.50±2.17; LNX2-shRNA: 37.24±5.56. Scale bars: 50 μm (A), 20 μm (B, C). Values are represented as mean±SEM, Student's t-test. **P<0.01. ***P<0.001. Color images available online at www.liebertpub.com/scd