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. 2015 Jul 14;10(7):e0132288. doi: 10.1371/journal.pone.0132288

Fig 1. BCG-pAg85A combination induces strong mycobacteria-specific proliferative and IFN-γ responses.

Fig 1

Evolution of Ag85A (Fig 1A) and PPD (Fig 1B) specific proliferation throughout the vaccination experiment in group 1 animals vaccinated with only BCG (black symbols), group 2 animals vaccinated with BCG-control vector (blue symbols) and group 3 animals vaccinated with BCG-pAg85A (green symbols). Each point represents mean cpm values of quintuplicate cultures of individual animals. Cells were stimulated in vitro with bovine PPD (M. bovis strain AN5, ex-Pasteur Institute of Brussels, 5 μg/mL final) or recombinant E. coli derived Ag85A (5 μg/mL final). Evolution of Ag85A (Fig 1C) and PPD (Fig 1D) specific IFN-γ production throughout the vaccination experiment. Bars represent mean IFN- γ levels ± SEM of six animals (pg/mL).