Table 3. SLA peptide affinity analysis.
| Peptide sequence | Ag85 | Amino acids | NetMHCpan Rank score a | SLA-1*0401 |
|---|---|---|---|---|
| (SLA-1*0401/SLA-1*0801) | affinity (nM) b | |||
| ASSALTLAI | A | 129–137 | 1.50/3.00 | 10 |
| SSALTLAIY | A | 130–138 | 0.40/0.50 | 17 |
| LAIYHPQQF | A | 135–143 | 7.00/4.00 | 20,000 |
| ASSALTLAIY | A | 129–138 | 0.25/0.40 | 1,971 |
| AIYHPQQFVY | A | 136–145 | 0.50/0.12 | 5 |
| LSMAGSSAM | B | 125–133 | 0.80/0.40 | 375 |
| SSAMILAAY | B | 130–138 | 0.20/0.17 | 106 |
| GSSAMILAAY | B | 129–138 | 0.40/0.30 | 7,755 |
| ILAAYHPQQF | B | 134–143 | 1.50/0.50 | 6,043 |
a: Peptides having NetMHCpan rank scores of <2.00 were considered as predicted for high affinity binding by the respective SLA molecules of interest (SLA-1*0401 and SLA-1*0801).
b: Binding affinity of the 9 synthetic peptides determined for SLA-1*0401 as described by Sylvester-Hvid et al [38]. Peptides with strong binding affinity are indicated in bold.