Skip to main content
. 2015 Jul 14;11(7):e1005048. doi: 10.1371/journal.ppat.1005048

Fig 2. Identification and characterization of Ostrinia Masc protein.

Fig 2

(A) Phylogenetic analysis of Ostrinia Masc protein. The neighbor-joining tree was generated using the amino acid sequences corresponding to the zinc finger domains from 43 proteins showing homology to B. mori Masc protein. The numbers on the internal branches represent the support value following bootstrap analysis (1,000 replicates). (B) Effect of Ostrinia Masc cDNA transfection on the Bmdsx splicing in BmN4 cells. The splicing patterns of Bmdsx in BmN4 cells transfected with Ostrinia or B. mori Masc expression vectors or empty vector were examined by RT–PCR. The F and M indicate female- and male-type splicing of Bmdsx, respectively. (C) Effect of Ostrinia Masc cDNA transfection on BmIMP expression. BmIMP expression was examined by RT-qPCR. rp49 was used as a normalization control. Data shown are means + standard deviation of triplicates.