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. 2015 Jul 14;10(7):e0132639. doi: 10.1371/journal.pone.0132639

Fig 6. Dysbindin-1A up-regulates the MMP-9 expression.

Fig 6

(A) NF-kappa B target gene products were examined in dysbindin-1A knockdown cells. Dysbindin-1A was silenced in N2a cells using si-dys2#, and mRNA levels of NF-kappa B downstream genes were examined using real-time PCR. The values shown represent the means ± S.E. of three independent groups. *, P<0.05; one-way ANOVA. (B) p65 overexpression restores gene expression resulting from dysbindin-1A deficiency. EGFP or EGFP-p65 was co-transfected with si-control or si-dys 2# into N2a cells. Forty-eight hours later, MMP-9 mRNA levels were detected using real-time PCR. The values shown represent the means ± S.E. (error bars) of three independent groups. **, P<0.01; EGFP/si-control vs. EGFP/si-dys; one-way ANOVA. ##, p<0.01; EGFP/si-dys vs. EGFP-p65/si-dys; one-way ANOVA. (C) NF-kappa B targeting gene products were examined in dysbindin-1A overexpressed cells. EGFP, dysbindin-1A-EGFP, dysbindin-1A-NLS-EGFP were transfected into N2a cells, the NF-kappa B downstream genes were examined using real-time PCR. *, P<0.05, **, P<0.01; EGFP vs. dysbindin-1A-NLS-EGFP; one-way ANOVA.