Table 1. Application scheme.
retinoic acid (RA) | BMP-4 | inhibitor / agonists / antagonists | |
---|---|---|---|
1 | 5 μM RA | - | - |
2 | - | 40 ng/ml BMP-4 | - |
3 | 5 μM RA | 40 ng/ml BMP-4 | - |
4 | 5 μM RA | - | 38 μM Boc-D-fmk a |
6 | 5 μM RA | 40 ng/ml BMP-4 | 38 μM Boc-D-fmk |
7 | - | - | 5 μM TTNPB b |
8 | - | 40 ng/ml BMP-4 | 5 μM TTNPB |
10 | - | - | 5 μM methoprene acid c |
11 | - | 40 ng/ml BMP-4 | 5 μM methoprene acid |
13 | 5 μM RA | - | 10 μM ER50891 d |
15 | 5 μM RA | 40 ng/ml BMP-4 | 10 μM ER50891 |
16 | 5 μM RA | - | 10 μM LE135 e |
18 | 5 μM RA | 40 ng/ml BMP-4 | 10 μM LE135 |
aBOC-D-fmk: caspase inhibitor
bTTNPB: RAR agonist
cmethoprene acid: RXR agonist
dER50891: RARα antagonist
eLE135: RARß antagonist.