Table 1. Primers used in the qRT-PCR experiments to measure gene expression levels of CRY1, CRY2, JHAMT, FAMeT, JHEH, CYP307A1 in 5th instar nymphs, and CRY1, CRY2, JHAMT, CYP307A1, Vg in macropterous virgin female adults of Sogatella furcifera.
| Gene | Description | Primer forward (Sequence 5' to 3') | Primer reverse (Sequence 5' to 3') | GeneBank Accession |
|---|---|---|---|---|
| 18S | Housekeeping genes | TGTCTGCTTAATTGCGATAACGAAC | CCTCAAACTTCCATCGGCTTG | JF773150.1 |
| RPL9 | Housekeeping genes | TGTGTGACCACCGAGAACAACTCA | ACGATGAGCTCGTCCTTCTGCTTT | From Guo-Qing Li |
| ARF | Housekeeping genes | CACAATATCACCGACTTTGGGATTC | CAGATCAGACCGTCCGTACTCTC | From Guo-Qing Li |
| CRY1 | Cryptochrome1 | CTGTTCTTCCAGCGGCAAC | TGCTCTCACTGCGTCTGTCC | From Guo-Qing Li |
| CRY2 | Cryptochrome2 | CCTTCTGCTATCACGTTTGCT | CACGCCAAATTATTTCAAGTTCGTC | From Guo-Qing Li |
| JHAMT | JH acid methyltransferase | TGAATTGACTGCCATTACGGTT | CAGTTGTGTTGTTCCCGCTCA | From Guo-Qing Li |
| FAMeT | Farsoic acid methyltransferase | TGAGTATAAGCCTTCAGTACCTAGC | GTTTCACAAGGCATTCCTCTCG | From Guo-Qing Li |
| JHEH | JH epoxide hydrolase | GCACTATAACATCTTCAATGCGACT | AACTCATTTGGGAATCTTGCACA | From Guo-Qing Li |
| Vg | Vitellogenin | CTGATCTGGCTTTCATAGCTCT | GCTGCCAACATGGATCAGAAC | From Guo-Qing Li |
| CYP307A1 | Cytochrome P450, family 307, subfamily A | GAGCCCAAAGACTTCACCGAT | CCAGCTCATAAAGAATGTGATGCC | KC701459.1 |