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. 2015 Jul 14;11(7):e1005382. doi: 10.1371/journal.pgen.1005382

Fig 9. Amu1 interacts with Mep1 and Mep3 in vitro.

Fig 9

After spheroplasting, the cells were lysed and Amu1-3HA was immunoprecipitated with anti-HA. Immunoblots of the lysates and immunoprecipitates (IP) with anti-HA and anti-GFP antibodies are shown. (a) Wt (23344c, negative control)) and AMU1-3HA (MB142) cells transformed with pGAL1Mep1-GFP, pGAL1Mep2N4Q-GFP or pGAL1Mep3-GFP were grown in the presence of proline (0.1%). Glutamine (gln, 0.1%) was added to AMU1-3HA (MB142) cells transformed with one of the 3 pGAL1Mep-GFP vectors (30 min). (b) Triple-mepΔ (31019b) cells transformed with pGAL1Mep1-GFP (negative control) and, triple-mepΔ amu1Δ (MB310) cells transformed with both pGAL1Mep1-GFP (LEU2) and YEpAmu1-HA (URA3) or with both pGAL1Mep1-GFP (LEU2) and YEpAmu14R-A-HA (URA3) were grown in the presence of proline (0.1%).