Fig 3. Promoter chromatin remodeling is not affected by lack of topoisomerases.

(A) ChIP analysis of Gal4 binding in the GAL gene promoters of wild type and top1Δtop2 ^ts cells following transcriptional activation. Experimental setup was as described for Fig 1B, and ChIP was performed using antibodies against Gal4. Gal4 binding levels in the GAL gene promoters were normalized to the binding under de-repressed conditions in wild type at the 0 min time point (set to 1). (B) ChIP analysis of nucleosome removal from GAL gene promoters of wild type and top1Δtop2 ^ts cells following transcriptional activation. ChIP was performed using antibodies targeting histone H3. H3 binding levels in the GAL gene promoters were normalized relative to the binding under uninduced conditions at the 0 min time point (set to 1). Averages from three (A) or two (B) individual experiments are shown, and error bars represent ± one standard deviation. Positions of primers used in the ChIP experiments for the individual GAL genes are indicated with arrows in Fig 1A and presented in Table 2.