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. 2015 Jul 14;10(7):e0132974. doi: 10.1371/journal.pone.0132974

Fig 1. Effect of pH on the motility and velocity of sperm.

Fig 1

Purified human sperm were incubated in pH 5.2, 6.2, 7.2 and 8.2 nutrient solutions for 15, 30, 60, 90 and 120 min, respectively. (A) Sperm mobility (PR + NP): a,b,e-g p<0.05, statistically significant different from pH 7.2 group at 15, 30, 60, 90, and 120 min, respectively; c p<0.05, statistically significant different from pH 6.2 group at 15 min; dp<0.05, statistically significant different from pH 5.2 group at 15 min. (B) PR: h,i,l-n p<0.05, statistically significant different from pH 7.2 group at 15, 30, 60, 90, and 120 min, respectively; jp<0.05, statistically significant different from pH 6.2 group at 15 min; kp<0.05, statistically significant different from pH 5.2 at 15 min. (C) mean straight line velocity (VSL): o-rp<0.05, statistically significant different from pH 7.2 group at 30, 60, 90 and 120 min, respectively; t p<0.05, statistically significant different from pH 6.2 group at 15 min; sp<0.05, statistically significant different from pH 5.2 group at 15 min. (D) mean curvilinear velocity (VCL): u-w p<0.05, statistically significant different from pH 7.2 group at 60, 90 and 120 min, respectively; y p<0.05, statistically significant different from pH 6.2 group at 15 min; xp<0.05, statistically significant different from pH 5.2 group at 15 min. and; (E) mean average path velocity (VAP): α, β, δ, ε, η p<0.05, statistically significant different from pH 7.2 group at 15, 30, 60, 90 and 120 min, respectively; θ p<0.05, statistically significant different from pH 6.2 group at 15 min; λp<0.05, statistically significant different from pH 5.2 group at 15 min (N = 3).