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. 2015 Jul 14;10(7):e0132949. doi: 10.1371/journal.pone.0132949

Fig 4. Stimulation of HEK-TLR2 cells transfected with an NF-κB-dependent ELAM-luciferase reporter gene with E. faecalis antigens.

Fig 4

HEK cells were stimulated for 6 h with escalating antigen concentrations as indicated, lysed and luciferase activity was determined by luminometry. The concentration of bacterial cells is expressed as dry weight per ml. Fold-induction denotes stimulated versus non-stimulated luciferase activity. Stimulation with whole bacterial cells (A). Stimulation with dialyzed cell-free culture supernatant (B). Stimulation with E. faecalis cell membrane total lipid extracts (C). Data are expressed as mean ± SEM of triplicates. ND = not detected. * p < 0.001 12030 ΔbgsA versus 12030 WT, ** p < 0.001 12030 ΔbgsA versus 12030 ΔbgsB, +++ p < 0.001 12030 ΔbgsB versus 12030 wild type by 2-way ANOVA with Bonferroni post-test for pairwise comparisons.