Table 4. Average Porcine Parvovirus (PPV) titres in inoculated bovine plasma used in Experiment 1 at each irradiation time (log10 values) 1 .
Time (min) | UV Irradiation Doses (J/l) | Mean of 3 replicates (log10 TCID50/mL 2 ) | Confidence interval | Standard deviation |
---|---|---|---|---|
0 | 0 | 5.20 | 0.13341 | 0.12 |
5 | 765 | 1.02 | 0.50306 | 0.44 |
10 | 1,530 | 0.15 | 1.11065 | 0.98 |
15 | 2,295 | Not detected 3 | ||
30 | 4,590 | Not detected | ||
45 | 6,885 | Not detected | ||
60 | 9,180 | Not detected |
1Liquid bovine plasma samples were passed three consecutive times in SK6 cells for PPV to amplify any viable virus which may have been undetected in the first passage.
2Tissue culture infection dose was determined by microtiter assay procedure [7].
3The theoretical limit of detection of the method used was estimated to be 0.23 viral particles per mL.