Table 1. Primers employed for qRT-PCR. DC-SCRIPT primers were designed in this study.
Gene | Abbreviation | Primer pair (RT-qPCR) |
---|---|---|
Elongation factor-1 α | ef1-α | F:AAATTGGCGGTATTGGAAC R:GGGAGCAAAGGTGACGAC |
18S ribosomial RNA | 18S | F:CGCCTGAATACCGCAGCTAG R:AGAACGGCCATGCACCACCAC |
α-tubulin | α-tub | F:GGCACTACACAATCGGCAAAGAGA R:TCAGCAGGGAGGTAAAGCCAGAGC |
Dendritic cell specific transcript | DC-SCRIPT | F:AACAGCACACGCTCACTCAC R:CGATCATGTGAGCCTTGAGA |