Figure 4.

Reproducibility of cell cycle lengths

1. “ABa” (top) and “ABp” (bottom) cell lineage trees derived from average cell cycle lengths of 91 wild-type embryos of approximately 350-cell stage with standard deviations indicated as red bars on division nodes (see “P1” lineage tree in Supplementary Fig S4A). Cell fates of “ABa” or “ABp” descendants are differentially color coded in a way similar to that in Fig1 (note some fates are labeled with extra depth here that is not observed in Fig1). Developing time in minutes is shown on the left starting from the last time point of “ABa” to the cutoff time point of 350-cell stage. Sister pairs used in screening for asynchrony are indicated with two-headed arrows that are color-coded in black, purple, and green to denote the following three types of division, respectively, that is, those giving rise to the same or different cell type(s) or one daughter to terminally differentiated cell and the other to a postembryonic blast cell (see also Fig1C). Parent of the sister cells is labeled with a numerical code corresponding to that in Fig5 below. Sublineages receiving Notch signaling (red dot) are indicated with two arrows and are highlighted in bold. Precursor of the excretory cell (ABplpapp) is indicated with an arrowhead. Precursor whose one daughter becomes terminally differentiated while the other develops into postembryonic blast cell is indicated with an arrow.
2. A heat map of mutual Pearson correlation's coefficient (r) of cell cycle lengths between 91 individual wild-type embryos. Both horizontal and vertical axes denote the coefficient of an individual embryo against another.
3. Distribution of sister-pair count based on their asynchrony (in minutes) in wild-type 350-celled embryos. Horizontal axis denotes asynchrony, and the vertical axis represents cell count. Asynchronies between sister pair that is longer than 5 min are shaded.