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. Author manuscript; available in PMC: 2016 Oct 1.

Published in final edited form as: Biochim Biophys Acta. 2015 Jan 14;1853(10 0 0):2802–2811. doi: 10.1016/j.bbamcr.2014.12.041

(A) C2C12 cells expressing Tet-inducible MitoTimer, exposed to Dox for 1 h, and then imaged at 4, 8, 12, 24, 48 and 72 h. Merged images of green and red channels are shown; smaller insets show the expression levels of MitoTimer green and MitoTimer red fluorescence. (B) Heterogeneous expression of MitoTimer fluorescence, indicating mitochondria with differential rates of import.

(A) C2C12 cells expressing Tet-inducible MitoTimer, exposed to Dox for 1 h, and then imaged at 4, 8, 12, 24, 48 and 72 h. Merged images of green and red channels are shown; smaller insets show the expression levels of MitoTimer green and MitoTimer red fluorescence. (B) Heterogeneous expression of MitoTimer fluorescence, indicating mitochondria with differential rates of import.