Table 1. Summary of TRP and Piezo channel mRNA expression.
| Gene |
Conventional PCR |
Single-cell RT–PCR |
Quantitative PCR |
CC | Brain | |
|---|---|---|---|---|---|---|
| ONH | ONH astrocyte | ONH | ONP | |||
|
TRPC channels | ||||||
| TRPC1 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPC2 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPC3 |
+ |
- |
+ |
nt |
nt |
nt |
| TRPC4 |
+ |
- |
+ |
nt |
nt |
nt |
| TRPC5 |
+ |
- |
+ |
nt |
nt |
nt |
| TRPC6 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPC7 |
- |
- |
nt |
nt |
nt |
nt |
|
TRPV channels |
|
|
|
|
|
|
| TRPV1 |
- |
- |
nt |
nt |
nt |
nt |
| TRPV2 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPV3 |
- |
- |
nt |
nt |
nt |
nt |
| TRPV4 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPV5 |
- |
- |
nt |
nt |
nt |
nt |
| TRPV6 |
- |
- |
nt |
nt |
nt |
nt |
|
TRPM channels |
|
|
|
|
|
|
| TRPM1 |
+ |
- |
+ |
nt |
nt |
nt |
| TRPM2 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPM3 |
+ |
- |
+ |
nt |
nt |
nt |
| TRPM4 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPM5 |
- |
- |
nt |
nt |
nt |
nt |
| TRPM6 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPM7 |
+ |
+ |
+ |
nt |
nt |
nt |
| TRPM8 |
- |
- |
nt |
nt |
nt |
nt |
|
TRPA channel |
|
|
|
|
|
|
| TRPA1 |
+ |
- |
nt |
nt |
nt |
nt |
|
TRPP channels |
|
|
|
|
|
|
| TRPP1 (PKD1) |
+ |
+ |
+ |
+ |
+ |
+ |
| TRPP2 (PKD2) |
+ |
+ |
+ |
+ |
+ |
+ |
| TRPP3 (PKD2L1) |
+ |
- |
nt |
nt |
nt |
nt |
|
Piezo channels |
|
|
|
|
|
|
| Piezo1 (Fam38a) |
+ |
+ |
+ |
+ |
+ |
+ |
| Piezo2 (Fam38b) | + | + | + | + | + | + |
All results were obtained from C57BL/6 mice or, in the case of the single cell RT–PCRs, from a transgenic, red fluorescent protein-expressing strain that is on the C57BL/6 genetic background. +, signal detectable; -, signal not detectable; nt, not tested; ONH, optic nerve head; ONP, optic nerve proper; CC, corpus callosum.