Table 2. Expression of TRP and Piezo channels in single ONH astrocytes (n=23).
| Gene | Number of astrocytes (%) | Gene | Number of astrocytes (%) |
|---|---|---|---|
| TRPC1 |
20 (87%) |
TRPP1 |
18 (78%) |
| TRPC2 |
5 (22%) |
TRPP2 |
18 (78%) |
| TRPC6 |
3 (13%) |
Piezo1 |
5 (22%) |
| TRPV2 |
3 (13%) |
Piezo2 |
10 (43%) |
| TRPV4 |
4 (17%) |
|
|
| TRPM2 |
1 (4%) |
|
|
| TRPM4 |
2 (9%) |
|
|
| TRPM6 |
7 (30%) |
|
|
| TRPM7 | 12 (52%) |
Single-cell RT–PCR was performed on individual astrocytes dissociated from the ONH of C57BL/6. 33 ONH astrocytes from five C57BL/6 mice were examined and only pure astrocytes (n=23) that were not contaminated with other cell components such as oligodendrocytes were used for gene expression analysis.