Table 1.
Round of panning |
Thio-PreS coupled beads |
Thioredoxin coupled beads |
||||
Phage applied | Phage eluted | EF1 | Phage applied | Phage eluted | EF | |
1st | 2.0 ± 1011 | 2.6 ± 104 | 1.3 ± 10-7 | 2.0 ± 1011 | 3.4 ± 104 | 1.7 ± 10-7 |
2nd | 1.0 ± 1011 | 1.2 ± 104 | 1.2 ± 10-7 | 1.0 ± 1011 | 9.5 ± 103 | 9.5 ± 10-8 |
3rd | 1.0 ± 1011 | 9.3 ± 104 | 9.3 ± 10-7 | 1.0 ± 1011 | 3.9 ± 104 | 1.2 ± 10-7 |
4th | 5.0 ± 1010 | 3.5 ± 105 | 7.0 ± 10-6 | 5.0 ± 1010 | 3.6 ± 104 | 7.2 ± 10-7 |
5th | 5.0 ± 1010 | 2.6 ± 106 | 5.1 ± 10-5 | 5.0 ± 1010 | 8.8 ± 104 | 1.8 ± 10-6 |
Enrichment factor (EF) = phage eluted/phage applied.