Figure 3.

Acute AIB hyperpolarization by a heterologously expressed HisCl1 histamine-gated Cl⁻ channel modulates both spontaneous and sensory-evoked behavior. A, B, Aversive responses to dilute 1-octanol without and with 4 mm HA (black and patterned bars, respectively) and spontaneous reversals were examined in wild-type and transgenic animals. HisCl1 cDNA was expressed in the AIBs under the inx-1 promoter in wild-type background. C, Aversive responses to dilute octanol in animals expressing HisCl1 selectively in the AIBs to varying concentrations of HA. Data represent mean ± SEM with 15–20 animals being tested in 3 independent experiments and were analyzed by two-tailed Student's t test with Welch's correction. *p < 0.001, significantly different from wild-type animals under identical conditions.