Figure 4.

The AWC sensory neurons are inhibited by 1-octanol and AWC glutamatergic signaling to the AIBs abolishes 5-HT-stimulated aversive responses. A, Trace average of octanol-evoked Ca²⁺i transients in ASHs and AWCs of wild-type animals and maximum octanol-evoked Ca²⁺i changes in ASHs of wild-type animals and AWCs of wild-type, unc-13, unc-31, and Psra-6::eat-4 RNAi animals. Data are presented as mean ± SEM. Numbers within bars indicate n. B, C, Aversive responses to dilute (30%) and 100% 1-octanol without and with food, 4 mm 5-HT and IAA (black, white, gray and checkred bars, respectively) were examined in wild-type,-null and transgenic animals. eat-4 was expressed in the AWCs using the nlp-1 promoter. glr-1 RNAi and glr-1 cDNA was expressed in the AIBs and AVA using the npr-9 and nmr-1 promoters, respectively. Data represent mean ± SEM with 15–20 animals being tested in 3 independent experiments and were analyzed by two-tailed Student's t test with Welch's correction. *p < 0.001, significantly different from wild-type animals under identical conditions.