Figure 5.

Activation of ASER modulates aversive responses. A, Aversive responses to dilute 1-octanol without and with 4 mm 5-HT (black and white bars, respectively) were examined in wild-type and transgenic animals. eat-4 was expressed in the ASEs, ASEL, and ASER using the flp-6, gcy-6, and gcy-5 promoters, respectively. eat-4 RNAi was expressed in ASER using the gcy-5 promoter. avr-14 and avr-14 RNAi was expressed in the AIBs using the inx-1 promoter. B, Salt down-steps were performed by incubating animals for 10 min on a high-salt plate (+10 mm) and then transferring back to a NGM plate to be assayed. Salt up-steps were performed by incubating on an NGM plate and transferring to a high-salt plate (+10 mm) for 10 min before assaying. Data represent mean ± SEM with 15–20 animals being tested in 3 independent experiments and were analyzed by two-tailed Student's t test with Welch's correction. *p < 0.001, significantly different from wild-type animals under identical conditions.