Fig. 2.

Chromosome copy number quantification by “agarose gel method”. a Agarose gel containing cell lysate samples of three independent cultures, in duplicate, flanked by a 1-kb GeneRuler DNA ladder. The white line indicates non-adjacent lanes of the same gel. The DNA concentration and chromosome copy number of each sample was quantified by comparing the fluorescence intensity of the gel bands to that of the left and right ladder. b The number of chromosome copies per cell. This was calculated by dividing the chromosome copy number of the lysate by the original cell density of each replicate