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. 2015 Jul 1;2015:429439. doi: 10.1155/2015/429439

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Copyright © 2015 Youhei Egami et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Live-cell imaging of GFP-ACAP2 and Rab35 in RAW264 macrophages during phagocytosis of zymosan. (a) RAW264 cells expressing GFP-ACAP2 were allowed to make contact with zymosan (arrows) and were observed by confocal laser microscopy. Phase-contrast images are shown (top panels). The elapsed time is indicated at the top. The binding of zymosan to the cell surface was set as time 0. Scale bar: 5 μm. (b) Live RAW264 macrophages coexpressing GFP-ACAP2 and TagRFP-Rab35 were incubated with zymosan and were monitored by confocal microscopy. Scale bar: 5 μm. Boxed area is enlarged (bottom left). Line scan analysis using MetaMorph program shows fluorescence intensities of GFP-ACAP2 (green) and TagRFP-Rab35 (red) at the position of the line in the enlarged image.