Figure 9. Concentration dependent oligomerization of PICK1^WT.

EOM on PICK1^WT at increasing protein concentrations; (A) 0.9 mg/ml (B) 2.3 mg/ml (C) 3.2 mg/ml (D) 5.3 mg/ml and (E) 7.5 mg/ml, including dimers, tetramers, hexamers and octamers in the generated pool. Top panels show D_max and R_g of generated pools in blue and selected pools in green. Bottom panels show SAXS data and fit of optimal ensemble. (F) Quantification of the fraction of dimers, tetramers, hexamers and octamers at different concentrations of PICK1^WT. (G) Quantification of the fraction of dimers, tetramers, hexamers and octamers at different concentrations of PICK1^LKV from analysis repeated with all species (see Figure S5 for analysis).