Figure 6.

Effect of RVT and 3,3′,4,4′-THS on proliferative capacity ((a)–(c)) and activity of SA-β-Gal ((d)–(g)) in ovarian cancer cells. The effectiveness of cancer cell replication and the activity of SA-β-Gal were measured for cells subjected to the tested compounds for 24 h using fluorescence-based techniques. Representative images show the expression of SA-β-Gal in A2780 cells exposed to 3,3′,4,4′-THS. The arrows indicate positive cells which were identified according to the presence of a blue precipitate within the cytoplasm (g). The asterisks indicate a significant difference as compared to the control group (Con). The hashes indicate a significant difference as compared to cells subjected to RVT. The experiments were performed in octuplicate.