Figure 2.

Immunodetection of PIP1 (A) and PIP2 (B) homologs in the root plasma membrane (PM) of Arabidopsis thaliana ecotype Col-0 wild type (WT), single (myb28) and double (myb28myb29) knockout mutants under non-saline (0 mM NaCl) and saline (100 mM NaCl) conditions. Total PM was separated by SDS-PAGE and probed with antibodies against AtPIP1 and AtPIP2. The PIP1 and PIP2 protein amounts (monomers and dimers) were quantified using the “Quantity one” program from Bio-Rad Laboratories. Mean values are shown (n = 3). Mean values with different letters represent significant (P < 0.05) differences according to the Tukey test. The scanned bands were normalized by calculating the ratio to the corresponding Coomassie-stained aquaporin band (29 KDa) ensuring that different intensities were not due to different loaded protein amounts.