Table 2. Summary statistics of Illumina sequencing results.
| 12° | 15° | 18° | 21° | 25° | |
|---|---|---|---|---|---|
| Raw sequences | 192,559,160 | 163,018,834 | 189,541,778 | 178,501,904 | 161,662,008 |
| Sequences after QC filtering | 182,390,970 | 154,294,234 | 179,245,463 | 167,554,816 | 152,818,985 |
| DE contigs upregulated | 4 | 104 | 697 | 3976 | |
| Upregulated contigs annotated by BLAST | 1 (25%) | 55 (53%) | 354 (51%) | 1902 (48%) | |
| Upregulated contigs assigned GO terms | 1 (25%) | 54 (52%) | 322 (46%) | 1608 (40%) | |
| Upregulated contigs assigned Enzyme Codes | 0 | 5 (5%) | 70 (10%) | 487 (12%) | |
| Upregulated contigs annotated by InterProScan | 1 (25%) | 50 (48%) | 347 (50%) | 1834 (46%) | |
| Upregulated KEGG pathways | 0 | 2 | 13 | 56 | |
| Upregulated KEGG Pathways with >2 enzymes | 0 | 0 | 5 | 25 | |
| DE contigs downregulated | 5 | 16 | 101 | 6166 | |
| Downregulated contigs annotated by BLAST | 2 (40%) | 5 (31%) | 40 (40%) | 3481 (56%) | |
| Downregulated contigs assigned GO terms | 2 (40%) | 3 (12%) | 31 (31%) | 2972 (48%) | |
| Downregulated contigs assigned Enzyme Codes | 0 | 0 | 8 (8%) | 626 (10%) | |
| Downregulated contigs annotated by InterProScan | 2 (40%) | 9 (56%) | 45 (45%) | — | |
| Downregulated KEGG pathways | 0 | 0 | 1 | — | |
| Downregulated KEGG pathways with >2 enzymes | 0 | 0 | 0 | — |
The number of contigs that were upregulated and downregulated at each temperature and were identified through a BLAST search of the NCBI database, assigned GO terms, assigned Enzyme Codes, or annotated through InterProScan. The percent of filtered sequences meeting each criterion follows in parentheses. Number of KEGG pathways for which an enzyme was differentially expressed is listed. *For two-fold change with a P < 0.01 and FDR of 0.10.