Table 3. Gene Ontology terms identified by Blast2GO analysis for contigs significantly upregulated at 25° (FDR 0.1, P < 0.01, >2-fold change).

GO Category	GO ID	Name	GO ID	Name	# Contigs
Biological Process	GO:0071840	Cellular component organization or biogenesis	GO:0016043	Cellular component organization	124
			GO:0007005	Mitochondrion organization	18
			GO:0006996	Organelle organization	50
	GO:0009987	Cellular process	GO:0007154	Cell communication	11
			GO:0007049	Cell cycle	87
			GO:0008219	Cell death	129
			GO:0019725	Cellular homeostasis	34
			GO:0006464	Cellular protein modification process	175
			GO:0007010	Cytoskeleton organization	85
			GO:0007165	Signal transduction	337
			GO:0006412	Translation	27
	GO:0032502	Developmental process	GO:0009653	Anatomical structure morphogenesis	202
			GO:0030154	Cell differentiation	152
			GO:0009790	Embryo development	87
			GO:0007275	Multicellular organismal development	315
	GO:0040007	Growth	GO:0016049	Cell growth	21
	GO:0008152	Metabolic process	GO:0009058	Biosynthetic process	155
			GO:0005975	Carbohydrate metabolic process	27
			GO:0009056	Catabolic process	170
			GO:0006259	DNA metabolic process	43
			GO:0006091	Generation of precursor metabolites and energy	11
			GO:0006629	Lipid metabolic process	68
			GO:0006139	Nucleobase-containing compound metabolic process	127
			GO:0044238	Primary metabolic process	6
			GO:0019538	Protein metabolic process	216
			GO:0019748	Secondary metabolic process	6
	GO:0051704	Multi-organism process	GO:0044403	Symbiosis, encompassing mutualism through parasitism	20
			GO:0016032	Viral process	20
	GO:0050789	Regulation of biological process	GO:0040029	Regulation of gene expression, epigenetic	4
	GO:0000003	Reproduction			91
	GO:0050896	Response to stimulus	GO:0007610	Behavior	33
			GO:0009628	Response to abiotic stimulus	51
			GO:0009607	Response to biotic stimulus	83
			GO:0009719	Response to endogenous stimulus	46
			GO:0009605	Response to external stimulus	100
			GO:0006950	Response to stress	237
	GO:0044699	Single-organism process	GO:0008283	Cell proliferation	91
			GO:0008037	Cell recognition	26
			GO:0007267	Cell–cell signaling	18
	GO:0006810	Transport	GO:0006811	Ion transport	52
			GO:0015031	Protein transport	66
Molecular Function	GO:0016209	Antioxidant activity			7
	GO:0005488	Binding	GO:0003779	Actin binding	34
			GO:0005509	Calcium ion binding	51
			GO:0030246	Carbohydrate binding	11
			GO:0003682	Chromatin binding	22
			GO:0008092	Cytoskeletal protein binding	20
			GO:0003677	DNA binding	142
			GO:1901363	Heterocyclic compound binding	1
			GO:0008289	Lipid binding	33
			GO:0003676	Nucleic acid binding	48
			GO:0000166	Nucleotide binding	262
			GO:0097159	Organic cyclic compound binding	1
			GO:0005515	Protein binding	498
			GO:0005102	Receptor binding	83
			GO:0003723	RNA binding	42
			GO:0008135	Translation factor activity, nucleic acid binding	5
	GO:0003824	Catalytic activity	GO:0016787	Hydrolase activity	98
			GO:0016301	Kinase activity	15
			GO:0003774	Motor activity	2
			GO:0004518	Nuclease activity	6
			GO:0008233	Peptidase activity	46
			GO:0004721	Phosphoprotein phosphatase activity	26
			GO:0004672	Protein kinase activity	58
			GO:0016740	Transferase activity	89
	GO:0009055	Electron carrier activity			19
	GO:0030234	Enzyme regulator activity			66
	GO:0060089	Molecular transducer activity	GO:0004871	Signal transducer activity	13
	GO:0001071	Nucleic acid binding transcription factor activity	GO:0003700	Sequence-specific DNA binding transcription factor activity	95
	GO:0004872	Receptor activity			43
	GO:0005198	Structural molecule activity			10
	GO:0005215	Transporter activity	GO:0005216	Ion channel activity	6
Cellular Component	GO:0030313	Cell envelope			1
	GO:0071944	Cell periphery	GO:0030312	External encapsulating structure	1
			GO:0005886	Plasma membrane	134
	GO:0005737	Cytoplasm	GO:0016023	Cytoplasmic membrane-bounded vesicle	68
			GO:0005829	Cytosol	134
			GO:0005811	Lipid particle	9
	GO:0005576	Extracellular region	GO:0005615	Extracellular space	38
			GO:0005578	Proteinaceous extracellular matrix	22
	GO:0043226	Organelle	GO:0005694	Chromosome	37
			GO:0005929	Cilium	4
			GO:0005856	Cytoskeleton	82
			GO:0005783	Endoplasmic reticulum	115
			GO:0005768	Endosome	19
			GO:0072546	ER membrane protein complex	1
			GO:0005794	Golgi apparatus	48
			GO:0005764	Lysosome	6
			GO:0005815	Microtubule organizing center	32
			GO:0005739	Mitochondrion	78
			GO:0000228	Nuclear chromosome	20
			GO:0005635	Nuclear envelope	14
			GO:0005730	Nucleolus	86
			GO:0005654	Nucleoplasm	166
			GO:0005634	Nucleus	233
			GO:0005777	Peroxisome	3
			GO:0005840	Ribosome	8
			GO:0005773	Vacuole	5
	GO:0043234	Protein complex			290

The three GO categories, Biological Process, Molecular Function, and Cellular Component, are presented along with GO terms of children and number of contigs assigned each term.