Figure 3.

Swi6 promotes boundary activity through the Epe1 pathway. (A) The swi6∆::kanMX mutation was introduced into reporter strains carrying IR-L or the IR-L B-box∆ mutation and boundary activity assayed by plate growth. (B) H3K9me chromatin immunoprecipitation enrichment over the ura4+ reporter gene for the strains assayed in A. Normalization as described in Figure 2C. Error bars represent the standard deviation. (C−D) The swi6Δ::leu1+ mutation was introduced into the reporter strain containing epe1Δ::kanMX and tested for boundary activity as in A-B. Error bars represent the standard deviation.