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Endogenous dafachronic acid (DA) concentrations in the wild type (WT), gon-2(q388), and glp-1(e2141) day-1 adults cultured at 20° or 25°. The DA concentrations were averaged over three independent experiments and normalized to level of the WT samples cultured at 20°. The absolute quantitation values are listed in Table S2. Error bars indicate the SD. Student’s t-tests were used for statistical analysis. n.s., not significant (P > 0.05).
