Figure 7.
Phosphatase null mutants show altered expression of iron-dependent genes during iron excess. The wild-type and the phosphatase null mutants were grown for 18 hr at 37° in AMM+ferrozine 300 μM+BPS 200 μM. Then, the mycelia were transferred to AMM+200 mM FeSO4 for 1 hr or 2 hr. The mycelium was harvested at the indicated times, and total RNA was extracted. The absolute quantitation of cccA (A), sreA (B), hapX (C), and the normalizer tubC, was determined by a standard curve (i.e., CT values plotted against a logarithm of the DNA copy number). The insets in (A) and (B) show the results of the wild-type. The results are the means (± SD) of three biological replicates (*P < 0.001, comparison of the null mutants with the wild-type strain).