Figure 6. TEPA inhibits migration, adhesion and invasion of MCF-7 cells.

(A) MCF-7 cells were incubated under control, 200 μM CoCl₂ or CoCl₂ with 50 μM TEPA treatment for 24 h, followed by a scratching assay. The relative wound closure was observed under microscope and photographed. Scale bars = 200 μm. (B) Quantification of cell motility by measuring the wound width. (C) After exposed to CoCl₂ or CoCl₂ with TEPA treatment for 24 h, MCF-7 cells were trypsinized and re-plated. Microscopic view of cells was taken after 6-h culture. Scale bars = 50 μm. (D) Quantification of cell adhesion. (E) Transwell assay of MCF-7 cells. Shown were representative fields of invasive and migratory cells on the membrane. Scale bars = 100 μm. (F) Quantification of cell invasion. All the experiments were performed using triplicate wells for each treatment condition and repeated three times. p < 0.05 was considered statistically significant (^*compared to the control group and ^#compared to the CoCl₂ group).