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. 2015 Jul 15;5:12410. doi: 10.1038/srep12410

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(A) Representative images of fluorescently stained actin of MCF-7 cells under control, 200 μM CoCl₂ or CoCl₂ with Ctr1 siRNA treatment. Scale bars = 10 μm. (B) Expression of E-cadherin in MCF-7 cells maintained for 24 h under control, 200 μM CoCl₂ or CoCl₂ with Ctr1 siRNA treatment by indirect immunofluorescence staining. Scale bars = 50 μm. (C) Expression of vimentin in MCF-7 cells maintained for 24 h under control, 200 μM CoCl₂ or CoCl₂ with Ctr1 siRNA treatment. Scale bars = 50 μm. (D) The fluorescence density of E-cadherin and vimentin per cell were measured in each groups. Data was shown as mean ± SD of three independent experiments. p < 0.05 was considered statistically significant (^*compared to the control group and ^#compared to the CoCl₂ group).