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. Author manuscript; available in PMC: 2016 Jan 14.
Published in final edited form as: Chem Commun (Camb). 2015 Jul 14;51(55):11019–11021. doi: 10.1039/c5cc02008e

Fig 3.

Fig 3

Analysis of the conjugate product of 2-OST and compound 1. Panel A shows the GPC-HPLC chromatogram of 2-OST protein only in the absence of compound 1. Panel B shows the GPC-HPLC chromatogram of compound 1 only. Panel C shows the GPC-HPLC chromatogram after incubating 2-OST with compound 1. Panel D shows the chromatogram of 2-OST and compound 3 after coupling reaction. Panel E shows the image of PAGE analysis of three proteins samples from GPC-HPLC analysis from Panel A-C. Panel F shows the results of the sulfotransferase activity measurement of three protein samples. The activity was determined based on the amount 35S-labeled sulfo group transferred to a polysaccharide substrate.