Fig. 2.

Vectors to investigate the endocytic pathway in Z. tritici. (A and B) Vectors for random ectopic integration of endocytic marker constructs into the genome of Z. tritici. The vectors pHFim1eGFP, pHeGFPRab5 and pHeGFPRab7 confer hygromycin resistance and are designed for random ectopic integration of GFP-marker fusion proteins into the genome of Z. tritici. Note that these vectors were derived from carboxin resistance conferring vectors. As such they contain part of the succinate dehydrogenase gene, carrying the mutation H267L and succinate dehydrogenase terminator. However, these fragments are of no significance. (C and D) Vectors for targeted integration of endocytic marker constructs into the sdi1 locus of Z. tritici. The vectors pCFim1eGFP, pCeGFPRab5 and pCeGFPRab7 contain the H267L point mutation in a stretch of sdi1 sequence, which confers carboxin resistance and allows targeted integration into the sdi1 locus of Z. tritici (for more information see Kilaru et al., 2015). Note that fragments are not drawn to scale. For more accurate information on fragment sizes see main text.