Table 2.
Primer name | Direction | Sequence (5′ to 3′)a |
---|---|---|
SK-Sep-14 | Sense | CATTTGCGGCTGTCTCGAAATCGACGGAAGGCAGTCGACGCCAGATGATGG |
SK-Sep-16 | Sense | ATGGTGAGCAAGGGCGAGGAG |
SK-Sep-42 | Antisense | CCACAAGATCCTGTCCTCGTCCGTCGTCGCTTACTTGTACAGCTCGTCCATGC |
SK-Sep-47 | Antisense | GGCGATGGTGGTATGCGGATG |
SK-Sep-61 | Sense | ATCACTCTCGGCATGGACGAGCTGTACAAGATGGCCGACGCCTCAGCTCCA |
SK-Sep-62 | Antisense | CCACAAGATCCTGTCCTCGTCCGTCGTCGCTCAACAAGCACATCCCTCCTTCG |
SK-Sep-63 | Sense | ATCACTCTCGGCATGGACGAGCTGTACAAGATGTCATCCAGAAAGAAGATCCTTT |
SK-Sep-64 | Antisense | CCACAAGATCCTGTCCTCGTCCGTCGTCGCCTAGCACGAGCAGCCTTGCTC |
SK-Sep-128 | Sense | CTCTCATAAGAGCTTGGCTGTCGACTCCTCGAATTCGAGCTCGGTACCCAACT |
SK-Sep-129 | Antisense | CTTTTCTCTTAGGTTTACCCGCGTTGAAGTGCGTTAACACTAGTCAGATCTACC |
SK-Sep-240 | Sense | CATCACTCACATCCGCATACCACCATCGCCATGAACGTGCTCAAGCTGCAGAAG |
SK-Sep-241 | Antisense | GGTGAACAGCTCCTCGCCCTTGCTCACCATACCCATCTTCTCCGACGCCGCC |
Italics indicate part of the primer that is complementary with another DNA fragment, to be ligated by homologous recombination in S. cerevisiae.