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. 2015 Jun;79:166–173. doi: 10.1016/j.fgb.2015.03.024

Fig. 3.

Fig. 3

Quantitative analysis of eGFP expression under inducible/repressible conditions. (A) Bar charts showing the extent of cytoplasmic fluorescence of eGFP in control cells and IPO323_CPnar1-eGFP in their sdi1 locus, both grown under repressed (OFF) and induced (ON) conditions. Mean ± standard error of the mean is shown, sample size n represents cells and is indicated. Double asterisk indicates significant difference at P = 0.004; triple asterisk indicates significant difference at P < 0.0001, Student t-test. Note that the data set represent measurements from two experiments and a single transformant. (B) Bar charts showing the extent of cytoplasmic fluorescence of eGFP in control cells and IPO323_CPlaraB-eGFP cells, both grown under repressed (OFF) and induced (ON) conditions. Mean ± standard error of the mean is shown, sample size n represents cells and is indicated. Triple asterisk indicates significant difference at P < 0.0001, Student t-test. Note that the data set represent measurements from two experiments and a single transformant. (C) Bar charts showing the extent of cytoplasmic fluorescence of eGFP in control cells and IPO323_CPex1AeGFP cells, both grown under repressed (OFF) and induced (ON) conditions. Mean ± standard error of the mean is shown, sample size n represents cells and is indicated. Triple asterisk indicates significant difference at P < 0.0001, Student t-test. Note that no green fluorescence was detected when expression of eGFP was controlled by the 1,4-β-endoxylanase A promoter (Pex1A; no difference to auto-fluorescence at P = 0.166; Student t-test). Note that the data set represent measurements from two experiments and a single transformant. (D) Bar charts showing the extent of cytoplasmic fluorescence of eGFP in control cells and IPO323_CPgal7eGFP, both grown under repressed (OFF) and induced (ON) conditions. Mean ± standard error of the mean is shown, sample size n represents cells and is indicated. Triple asterisks indicate significant difference at P < 0.0001, Student t-test. Note that the promoter allows weak expression of eGFP under repressed conditions. Note also that the data set represent measurements from two experiments and a single transformant. (E) Bar charts showing the extent of cytoplasmic fluorescence of eGFP in control cells and IPO323_CPicl1eGFP, both grown under repressed (OFF) and induced (ON) conditions. Mean ± standard error of the mean is shown, sample size n represents cells and is indicated. Triple asterisks indicate significant difference at P < 0.0001, Student t-test. Note that the promoter allows considerable expression of eGFP under repressed conditions. Note also that the data set represent measurements from two experiments and a single transformant. (F) Bar chart showing the relative cytoplasmic fluorescence of eGFP in all promoter strains under induced conditions. All intensities were normalised against eGFP fluorescence, driven by the α-tubulin promoter Ptub2.