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. 2015 Jun;79:158–165. doi: 10.1016/j.fgb.2015.04.014

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Localization of polarity markers in hyphal tips of Z. tritici. (A) Plate growth assay of IPO323 (WT), IPO323_HeGFPRab11 (Rab11), IPO323_CZtGFPSec4 (Sec4), IPO323_CZtGFPExo70 (Exo70), IPO323_CZtGFPMlc1 (Mlc1) and IPO323_CZtGFPSpa2 (Spa2). Colonies were grown for 5 days. No difference was found between all strains, suggesting that expression of the fluorescent markers is not affecting growth on solid media. (B) Localization of all florescent markers in hyphae of strains IPO323_CZtGFPSpa2 (Spa2), IPO323_CZtGFPExo70 (Exo70), IPO323_CZtGFPSec4 (Sec4), IPO323_CZtGFPMlc1 (Mlc1), and IPO323_HeGFPRab11 (Rab11). Fluorescent signals and corresponding DIC images were overlaid. Bar represents 5 micrometers. (C) Pseudo-color images of fluorescent signals off all marker proteins in hyphal tips. Note that Sec4, Mlc1 and Rab11 are focused in a single spot, whereas Spa2 and Exo70 form an apical cap. Signal intensities are provided in a color code (white = very strong signal, purple = weak signal). Bar represents 2 micrometers.